JGV Direct - Antisense RNA of hepatitis B virus (K. Moriyama and others)

								ARTICLE ABSTRACT
								DOI: 10.1099/vir.0.19170-0
								Online 1 May 2003

Antisense RNAs transcribed from the upstream region of the precore/core promoter of hepatitis B virus

Kosei Moriyama,¹ Kazuhiro Hayashida,² Mitsuo Shimada,³ Shuji Nakano,² Yoshiyuki Nakashima¹ and Yasuyuki Fukumaki⁴

¹Department of Medicine, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan
²Department of Medicine and Biosystemic Science, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan
³Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan
⁴Division of Disease Genes, Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan

The bidirectional activity of the precore/core promoter of hepatitis B virus (HBV) has been demonstrated in cultured cell lines. However, HBV antisense transcripts (asRNAs) have not been demonstrated in vivo. In the present study using liver tissue from patients with chronic hepatitis, an anchored 5´RACE mapping the 5´ ends at position 1680/1681, 1655 or 1609/1602 was carried out. In limited cases, RLM-3´RACE detected asRNAs to terminate at four or five consecutive dT residues in the 0.7 kb downstream region. PCR of oligo(dT)-primed cDNA did not amplify a typical polyadenylated asRNA. RT-PCR using various primers did not detect any spliced forms. Competitive RT-PCR estimated the copy numbers of the asRNAs to be 0.05-0.4 % of total sense RNAs. All sequenced asRNAs had ORF6 but, in one patient, the asRNA initiating at position 1680/1681 had additional initiation and termination codons in front of ORF6. Therefore, asRNAs are transcribed by RNA polymerase III at a low level, encompass a dispensable ORF6 gene and might be retained in the nucleus. The endogenous asRNAs complementary to the common ends of all sense RNAs suggest antisense-mediated self-regulation of hepadnavirus.

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This article is now available in the July 2003 print issue of JGV (vol. 84, 1907–1913). The complete issue of the journal may be seen in electronic form on JGV Online.