Journal of General Virology

Fig. 5. Regulation of Dlg stability in the absence of E6. U2OS cells were stably transfected with either pCDNA-3 alone (C) or the indicated Dlg mutant derivatives. Cell lines were then incubated in the presence (+) or absence (–) of the proteasome inhibitor CBZ for 2 h. After this time, cells were harvested and levels of Dlg mutant protein expression determined by Western blotting using an anti-HA mAb. The positions of the respective mutants are indicated by arrows.

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