Journal of General Virology

Fig. 3. Immunoblot analysis of pooled cell lysates (1x10⁶ cells per lane) from SAF-treated rats confirmed the presence of PrP^Sc in lymph DCs (lane 4) but not in T or B lymphocytes. SAF equivalent to 2 or 4 µg of infected brain tissue was loaded in lanes 5 and 6, respectively. Treatment of samples in the presence (+) or absence (–) of proteinase K (PK) before electrophoresis is indicated. PrP dimers are seen at approximately 60 kDa in lanes 3 and 4.

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