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Fig. 2

Fig. 2. ELISA detection of PRSV HA (filled triangle ) and YK (filled square ) and recombinants YK-AS/EN (x) and YK-AE (filled diamond) in leaves of infected non-transgenic papaya. Samples were collected at periodic intervals from the same three leaves of test plants and tested by ELISA. Sampled leaves were positioned as the top expanded leaf at 8 (A), 12 (B), and 14 (C) days p.i. The results are the mean ELISA readings of four or five plants from two experiments. filled circle , Healthy non-transgenic papaya. The readings were taken after 1 h of substrate hydrolysis.

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