Journal of General Virology

Fig. 6. Cryoimmuno EM analysis of DMVs formed upon nsp2–3His expression. Cells were fixed at 8 or 12 h post-transfection. Bars, 100 nm. (A) Labelling by using the anti-nsp2 antiserum of a DMV still connected to closely apposed ER membranes (arrows). (B) Tightly apposed ER membranes, a DMV connected to the ER and a 'mature' DMV, all labelled with the anti-nsp2 antiserum (arrows). (C) Labelling with the anti-nsp3 antiserum, demonstrating that nsp3 localizes to the DMVs in nsp2–3-expressing cells (big arrow). The small arrow indicates a region of the DMV where the inner and outer membranes are continuous, creating a connection between the DMV interior and the cytoplasm.

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