Journal of General Virology

Fig. 7. Distribution of vimentin, plectin and tubulin in COS-7 cells expressing the different vif gene products. (A–C), (E–G) and (I–K) show indirect double immunofluorescence for Vif protein (shown alone in A, E and I), vimentin (shown alone in B), plectin (shown alone in F) and tubulin (shown alone in J). (C), (G) and (K) show the merged images of (A) plus (B), (E) plus (F), and (I) plus (J). VifWt is shown in these images. However, both VifA45 and VifA45open show similar distributions and similar effects on vimentin, plectin and tubulin as VifWt and are therefore not shown. The negative controls in (D), (H) and (L) of cells transfected with pNL-A1-Vif-minus are merged images after double-staining with anti-HIV (red fluorescence) plus anti-vimentin (green fluorescence) (D), anti-plectin (H) and anti-tubulin (L), respectively. All transfected cells were examined at 40 h p.t.

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