Journal of General Virology

Fig. 4. Nested PCR for specific detection of ElHV-1. Heart and liver samples from Kiba (lanes 2 and 3) and serum samples from Kiba (lanes 4), Plai Kiri (5), Plai Kiri's mother Pang-Pha (6) and several other members of the Asian elephant herd (Ayesha, Svea, Iyoti and Drumbo; lanes 7–10) of the Berlin zoological gardens were analysed with nested PCR with the primer pair El_DPOL_2s (sense) plus El_DPOL_2as (anti-sense) in first-round PCR (a) and the pair El_DPOL_3s (sense) plus El_DPOL_3as (anti-sense) in second-round PCR (b). Water without template served as a control (lanes 11). A 100 bp ladder (Boehringer Mannheim) (size of prominent band, 0.5 kbp) was used as a size marker (lanes 1 and 12).

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