Journal of General Virology

Fig. 6. Kinetics of incorporation into MHV RI/RF and subgenomic TI/TF RNA size classes. Infected cells were labelled at 6.5 h p.i. with [³H]uridine for 2–90 min periods, as indicated. Cell lysates were treated with RNase T1 to obtain the RF/TF cores, which were separated by electrophoresis on 1 % agarose gels. The area of the gels containing each RF/TF species (, RF; , TF-II; , TF-III; , TF-IV; , TF-V; , TF-VI; , TF-VII) was cut out and its amount of radiolabelled RNA was determined by scintillation spectroscopy.

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