Journal of General Virology

Fig. 6. Expression of GFP–EBNA-5 does not influence the proteasome inhibitor-induced nuclear and nucleolar accumulation of wild-type p53 in MCF-7 cells. MCF-7 cells, transfected with the GFP–EBNA-5 construct, were cultured 1 day after transfection in the presence of DMSO (a, c, e) or 10 µM of MG132 (b, d, f) for 16 h, and analysed for p53 expression by immunostaining. Panels a and b, GFP–EBNA-5 and DNA; panels c and d, wild-type p53; panel e, phase contrast of the field shown in panels a and c; panel f, phase contrast of panels b and d. Arrow indicates cell with GFP–EBNA-5 in the nucleoli.

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