Journal of General Virology

Fig. 4. Analysis of the deletion mutants. (A) The top line represents the physical map of the wt SeMNPV genome for PstI restriction endonuclease. A 10593 bp fragment, including egt, v-cath, gp37, chiA, etc. (corresponding to nt 18513 to 29106; dashed horizontal line), was deleted in the recombinant SeXD1. The deletion in a genotypic variant of wt SeMNPV was also from nt 18513 to nt 29106 (a total of 10593 bp). (B) PstI restriction endonuclease analysis of genomic DNAs from wt SeMNPV and SeXD1. The PstI D fragment was absent in SeXD1. (C) PCR analysis of genomic DNAs from wt SeMNPV and SeXD1. The primers A and B correspond to nt 17874 to 17904 and 29135 to 29163, respectively.

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