Journal of General Virology

Fig. 1. Expression of pcDNA3.1/P1–2A3C3D. (a) Lanes: 1, COS-7 cells infected with vaccinia virus vTF7-3 (negative control); 2, cells transfected with pcDNA3.1/P1–2A3C3D and co-infected with vTF7-3; 3, uninfected cells transfected with pcDNA3.1/P1–2A3C3D; 4, cells transfected with pcDNA3.1 (negative control). The cells were labelled for 24 h (as described in Methods) with [³⁵S]Promix and cell extracts were immunoprecipitated with guinea pig anti-FMDV antiserum. (b) Uninfected COS-7 cells were transfected with pcDNA3.1/P1–2A3C3D and stained with anti-VP1 MAb B2, as described in Methods.

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